Tuesday, March 08, 2005

Southern blotting

I could have sworn the oligos for Southern blotting were made of RNA, but that doesn't really make sense in retrospect. I looked it up:

The basics of Southern blotting (from MBOC 4):

Southern blotting is used to analyze DNA as opposed to its analagous method of analyzing RNA, Northern blotting. Your sample (DNA or RNA) is resolved using gel electrophoresis, and transferred to a membrane (now called the blot) either by electrotransfer or by stacking piles of heavy books on top of the blot allowing for passive transfer. In both Northern and Southern blotting, single-stranded DNA oligonucleotide probes are hybridized to a target sequence in your sample. Depending on the variety of label is present in your oligonucleotide probe, the bands on the blot are visualized using autoragiography or by chemical means.

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